Dr. Sarah Stanley and Dr. Alexandra Alvarsson discuss the use of whole-organ imaging of the pancreas to reveal close interactions between nerves and islets and dynamic regulation of islet innervation in diabetes. In particular, they provide an overview of their methodology, which provides detailed information and quantification of pancreatic innervation in healthy pancreas, in canonical models of diabetes and in samples from nondiabetic and diabetic donors. They present their findings, demonstrating greatly enriched innervation in the islets with regional variations. They also discuss beta cell innervation in mouse models of diabetes and in pancreata from human donors with type 2 diabetes.